Available courses

The text combines explanations of the statistical foundation of data mining with useful software so that the tools can be readily applied and put to use. The series of lectureswill  give a deeper description of the methods, and a complete guide to computing with R.

UNIT-I: MICROBIAL KINETICS

Basic rate expressions; parameter values; basic mass balances; soluble microbial products;nutrients and electron acceptors; inhibition

UNIT-II: REACTORS

Reactor types: suspended growth reactors; batch reactor ; continuous -Flow Stirred Tank Reactor with Effluent Recycle; membrane reactors; rotating drum reactors; biofilm reactors; reactor arrangements; Mass Balances ; Reactors with Recycleing of Settled Cells- CSTR with Settling and Cell Recycling Evaluation of assumptions; Engineering Design of Reactors; Reactors in series

UNIT-III: BIOFILM KINETICS

Microbial aggregation; Why biofilms?; The idealized biofilm – substrate phenomenon- the biofilm itself; The steady state biofilm; The steady-State- Biofilm Solution; Estimating parameter values; average biofilm SRT; soluble microbial products and inert biomass;

UNIT-IV: ADVANCED MICROBIAL REACTORS FOR WASTE WATER

TREATMENT-Case study of microbial bioreactors for following types of waste water treatment: Municipal,beverage industry effluent, dairy effluent.

This Course will comprise of 20 lectures and would draw largely on bioperl programming, for Molecular Biological processes..It will also talk about filogenetic analysis and would have a section on analytics using R language..

UNIT –I: BASIC LABORATORY INSTRUMENTS

Buffers: Henderson and Hesselbach equation, pka and pkb. Preparation of Buffers.Viscosity: specific, intrinsic and reduced viscosities, viscometers, determination of molecular size and shape through viscosity.

Osmosis: osmosis in relation to molecular size and molecular weight, osmometer, partial, specific volume and diffusion co-efficient, Dialysis, membrane filtration and its application.

Principle and working of: pH meter, Sterilization techniques

UNIT – II: SEPARATION TECHNIQUES

Centrifugation: Types of centrifuge machines, analytical centrifuges, differential centrifuge, and Ultracentrifuge. Basic principles and applications: of gel filtration chromatography, ion exchange and affinity chromatography, gas liquid chromatography (GLC), high pressure/performance liquid chromatography (HPLC). Basic principles of Electrophoresis, Agarose gel and SDS- PAGE, isoelectric focusing, 2D Electrophoresis.

UNIT -III: SPECTROSCOPY, RADIOGRAPHY AND TRACER TECHNIQUES

Spectroscopic techniques: theory and applications of UV, Visible, IR, NMR, Fluorescence, AAS and AES. Principles and applications of tracer techniques in biology: Radioactive isotopes and half life of isotopes, Effect of radiation on biological system; Autoradiography, Cerenkov radiation, Liquid scintillation counter, Dosimetry. Laboratory procedures and safety aspects.

UNIT -IV: MICROSCOPY AND STAINING OF MICROORGANISMS

Microscopy: Principles and microscope application of bright-field and dark-field microscopy, Phase- contrast microscopy, fluorescence microscopy, Electron Microscopy : TEM, SEM and AFM , Confocal microscopy Cytophotometry and flow cytometry. Fixation and staining: Simple staining, negative staining, gram staining, acid fast staining, structural stains (Endospore, capsule and flagella).

UNIT-I: INTRODUCTION TO DNA

Nucleic acids- Structure and properties, Repetitive DNA, DNA renaturation kinetics. Forms of DNA and DNA supercoiling.DNA replication in prokaryotes: creation of replication fork, various models of DNA replication, enzymes involved in DNA replication.


Prokaryotic and Eukaryotic gene structure, , Transcription machinery, mRNA processing;

Reverse transcription; Genetic code and mechanism of translation, Post-tranlational modifications in proteins. Signal sequences and protein transport.


UNIT-II: Gene Regulation

Covalent modification of chromatin, Histone code hypothesis, Chromatin remodeling complexes, heterochromatization, Regulation of gene expression in bacteria (lac operon, trp operon) and eukaryotes; RNA dependent gene silencing.


UNIT-III: DNA MUTATIONS

Types of mutations, molecular basis of spontaneous and induced mutations, various physical and

chemical mutagenic agents, site directed mutagenesis, DNA repair mechanisms. Ames test;

Environmental mutagenesis and toxicity testing; Induction of mutation in Neurospora crassa and yeast, cytoplasmic inheritance and biochemical mutants. Transposable elements and mechanisms of transposition. Uses of transposons in genetic analyses.


UNIT- IV: BACTERIAL RECOMBINATION

Bacterial Gene Transfer: gradual development of the concept, Transduction, complementation, Conjugation; Genetic recombination- Bacteriophages; synapsis of homologous duplexes,

breakages and re-union; role of Rec A in recombination; Legitimate and illegitimate recombination, gene conversion; Bacterial transformation, Host cell restriction, & Transfection


MICB-803E2: MICROBIAL BIOREACTORS

UNIT-I: MICROBIAL KINETICS

Basic rate expressions; parameter values; basic mass balances; soluble microbial products; nutrients and electron acceptors; inhibition


UNIT-II: REACTORS

Reactor types: suspended growth reactors; batch reactor ; continuous -Flow Stirred Tank Reactor with Effluent Recycle; membrane reactors; rotating drum reactors; biofilm reactors; reactor

arrangements; Mass Balances ; Reactors with Recycleing of Settled Cells- CSTR with Settling and Cell Recycling -Evaluation of assumptions; Engineering Design of Reactors; Reactors in series


UNIT-III: BIOFILM KINETICS

Microbial aggregation; Why biofilms?; The idealized biofilm – substrate phenomenon- the biofilm itself; The steady state biofilm; The steady-State- Biofilm Solution; Estimating parameter values; average biofilm SRT; soluble microbial products and inert biomass;


UNIT-IV: ADVANCED MICROBIAL REACTORS FOR WASTE WATER TREATMENT


Case study of microbial bioreactors for following types of waste water treatment: Municipal, beverage industry effluent, dairy effluent.


We shall be using Arduino along with breadboard to show how to set up temperature, humidity, ac controller, GPS setting measuremenrs